FIGURE 1.

Rac1b has altered binding affinity to common regulators of Rho family GTPases. A, silver-stained protein gel after SDS-PAGE of protein complexes purified from cellular lysates shows proteins that differentially interact with YFP-Rac1b, YFP-Rac1, and YFP-Rac1V12. YFP used as a negative control for protein interactions. B–D, peptide coverage maps of GIT1 (B), RhoGDI (C), and SmgGDS (D). For B–D, the peptides used for identification are underlined and in boldface type. E, Western blot (WB) of total lysates from cells transiently transfected with YFP-Rac1b, YFP-Rac1, and YFP-Rac1V12 shows expression levels of GIT1, RhoGDI, PAK, YFP-Rac1, YFP-Rac1V12, YFP-Rac1b, and YFP as a control. IP, immunoprecipitation.