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p120ctn does not inhibit intrinsic GDP/GTP exchange activity of Rac1b. A, p120ctn isoform 1 inhibits Dbs (0.8 μm)-stimulated GDP/GTP exchange of RhoA, tested at 1 and 2 μm (2×). B, biochemical GEF exchange assay for Rac1b shows that neither p120ctn isoform 1 nor p120ctn isoform 4 inhibits Rac1b intrinsic GDP/GTP exchange activity. p120ctn isoform 1 was used at 0.6 and 2 μm (3×); p120ctn isoform 4 was used at 1 and 3 μm (3×).
