FIGURE 2.

Western blot analysis of STIM1, Orai1, and Orai3 expression in breast cancer cells. A, all 10 breast cancer cells lines were lysed, and 50 μg of proteins each were loaded in the same gels. After transfer, membranes were probed with specific antibodies against either STIM1, Orai1, or Orai3 followed by the appropriate secondary antibody coupled to peroxidase as described under “Materials and Methods,” and proteins bands were visualized using the ECL kit. B, blots obtained from 3–4 independent experiments were analyzed using Image J software and densitometric ratios to corresponding actin were calculated. All densitometric values were then normalized to those of the normal cell line MCF10A.