FIGURE 2.

Aggregation kinetics of recombinant mouse PrP monitored by thioflavin T fluorescence. Fluorescence was measured for 18 h as described under “Experimental Procedures.” The PrP concentration was 5 mg/ml in 20 mm sodium acetate, pH 3.5, in the presence of 100 mm NaCl and 10 μm ThT. Aggregation kinetics of PrP alone (empty circles) (A and B), or in the presence of C1q (A), or C1q globular domain (B) at varying concentrations: 0.2 mg/ml (open triangles), 0.3 mg/ml (inverted open triangles) and 0.4 mg/ml (open diamonds). C1q and its globular domain alone do not bind to ThT (open squares) (A and B). All experiments were performed at 40 °C in triplicate using a microplate reader. RFU, relative fluorescence units.