Analysis by size-exclusion chromatography of PrP aggregation reaction time points. PrP alone (line) and PrP in presence of 0.4 mg/ml of C1q (dashed line) aggregations are analyzed by SEC over time. A, 1 h after aggregation. B, 4 h after aggregation. C, 6 h after aggregation. I′, oligomer I′; II, oligomer II; M, monomer. Incubation was performed at 40 °C. mAu, milliabsorbance units.