FIGURE 7.
Oligomer II is toxic to cultured cells. Neuronal cells from PrP+/+ or PrP0/0 mice were exposed for 24 h to purified PrP isoforms ranging at 1 μm (white bars), 1.5 μm (gray bars) and 2 μm (black bars). Cell viability was then measured using the WST-1 assay. A, PrP+/+ N2aD11 cells. B, PrP0/0 NplI hippocampal cells. Untreated cells (hatched bars) and cells treated with equivalent volumes of vehicle buffer were used as controls. Statistical analysis was performed using Student's t test with Welsh correction. Error bars represent the means +/− S.E. of three independent experiments.