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. 2010 Apr 27;285(25):19316–19323. doi: 10.1074/jbc.M110.130716

FIGURE 2.

FIGURE 2.

Wild-type and Cys-less sialin exhibit similar transport kinetics. A, time course of 3H-NANA uptake (30 nm final concentration) by wild-type (WT) sialin- (filled circles, solid line) and Cys-less sialin- (open circles, dashed line) transfected cells. NEM treatment (2 mm, pH 7.5 for 5 min at 23 °C) inhibits NANA uptake (30 nm final concentration, measured at 5 min) into cells transfected with the wild-type protein, but not Cys-less-transfected cells (inset). B, wild-type- and Cys-less sialin-transfected cells exhibit saturable NANA uptake. Measurement of NANA uptake at 2 min in the presence of increasing substrate concentrations reveals KmNANA values of 4 ± 2 and 5 ± 2 mm for wild-type and Cys-less sialin, respectively, with a VmNANA of ∼5 nmol/min per well for both. ***, p < 0.0001; ns, no significance.