TABLE 1.

Sumoylated peptides observed in vivo by cysteine tag peptide enrichment and LC-MS/MS

Swiss-Prot accession number	Entry name	Protein name	SUMO site	Peptide sequencea	QSTARb		LTQ Orbitrapc
					Mascot score	Expectation value	Mascot score	Expectation value
P46060	RAGP1_HUMAN	Ran GTPase-activating protein 1	Lys524	516LLVHMGLL(GG-)KSEDK528	16.1	1.5	29.5	0.011
			Lys8	Ac-2ASEDIA(GG-)KLAETLAK15			48.5	0.00025
Q13263	TIF1B_HUMAN	Transcription intermediary factor 1-β	Lys779	775LTED(GG-)KADVQSIIGLQR790	28.2	0.21	41.6	0.0012
P63165	SUMO1_HUMAN	Small ubiquitin-related modifier 1	Lys7	Ac-2SDQEA(GG-)KPSTEDLGDKK17	43.0	0.012	32.5	0.026
			Lys25	24L(GG-)KVIGQDSSEIHFK37			30.2	0.018
P61956	SUMO2_HUMAN	Small ubiquitin-related modifier 2	Lys11	8EGV(GG-)KTENNDHINLK21	14.3	6.6	16.8	0.72
Q16666	IF16_HUMAN	γ-Interferon-inducible protein Ifi-16	Lys561	560L(GG-)KTEPEEVSIEDSAQSDLK578			58.9	0.00006
Q9UPN6	RBM16_HUMAN	Putative RNA-binding protein 16	Lys18	6pTFNSELYSLNDY(GG-)KPPISKAK25			37.0	0.012
Q9UG01	IF172_HUMAN	Intraflagellar transport protein 172 homolog	Lys4	Ac-1MHL(GG-)KHLR7			30.0	0.014
Q99518	FMO2_HUMAN	Dimethylaniline monooxygenase (N-oxide-forming) 2	Lys492	486NAIFTQ(GG-)KQRILKPLK500			16.3	0.023
Q96QD9	FYTD1_HUMAN	Forty-two-three domain-containing protein 1	Lys140	139R(GG-)KANLLR145			22.8	0.028
Q9UBD0	HSFX1_HUMAN	Heat shock transcription factor, X-linked	Lys215	211RVGV(GG-)KSAPR219			21.1	0.036
Q9UBG0	MRC2_HUMAN	C-type mannose receptor 2	Lys1142	1139LLQ(GG-)KPLR1145	21.9	0.043
Q8IYA6	CKP2L_HUMAN	Cytoskeleton-associated protein 2-like	Lys198	198(GG-)KPDPKLpYTR206	26.8	0.33	17.9	0.36

^a (GG-)K, diglysyl Lys (sumoylation site); Ac-, acetylated protein N terminus; pT/pY, phosphorylated Thr/Tyr.

^b Mass tolerances for precursor and fragment ions were 0.05 and 0.15 Da, respectively.

^c Mass tolerances for precursor and fragment ions were 5 ppm (Orbitrap) and 0.8 Da (LTQ), respectively.