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pH dependence of kobs for oxygen rebinding to the dodecameric form of C. aestuarii Hc (circles) and CaeSS2 monomer (squares) in the presence (red) and absence (blue) of 40 mml-lactate. Experimental conditions: 20 μm (subunit) Hc; 1.39 mm O2; laser pulse, 40 mJ, 10 Hz; 20 °C. Each trace is an average of 64 × 8 or 64 × 10 shots.
