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. 2010 Apr 21;285(25):19346–19353. doi: 10.1074/jbc.M110.116749

FIGURE 6.

FIGURE 6.

The interactions among Bem1 SH3b-CI, Cdc42, and Ste20 CRIB. A, His-Cdc42 (1–179, Q61L) or His-Cdc42 (1–191,Q61L) was incubated with GST-Bem1 SH3b-CI in the presence or absence of Ste20 CRIB. The proteins pulled down with glutathione-Sepharose 4B were subjected to SDS-PAGE and stained by immunoblotting using appropriate antibodies. B, overlay of 1H-15N HSQC spectra of 15N-Bem1 SH3b-CI (red), 15N-Bem1 SH3b-CI in the presence of unlabeled Cdc42 (blue), and 15N-Bem1 SH3b-CI in the presence of both unlabeled Cdc42 and Ste20 CRIB (green) are shown. Chemical shift changes of Ala-186 and Ala-247 are shown in the insets.