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. 2010 Apr 19;285(25):19372–19383. doi: 10.1074/jbc.M110.112896

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — Analysis of ligand binding by monitoring changes in intrinsic tryptophan fluorescence in the presence of all-trans-retinal. Data obtained from the fluorescence emission spectra of wild type and mutant ECD2 polypeptides described in Fig. 7 were analyzed in terms of percent change in intensity versus log of the all-trans-retinal concentration. , ECD2 WT; ▾, R1443H; □, W1408L; ◇, C1488R.

Inline graphic — Analysis of ligand binding by monitoring changes in intrinsic tryptophan fluorescence in the presence of all-trans-retinal. Data obtained from the fluorescence emission spectra of wild type and mutant ECD2 polypeptides described in Fig. 7 were analyzed in terms of percent change in intensity versus log of the all-trans-retinal concentration. , ECD2 WT; ▾, R1443H; □, W1408L; ◇, C1488R.