TABLE 3.

Summary of secondary structure analysis and binding constants for the ABCA4-ECD2 WT and mutant polypeptides

Genotype	α-Helix	β-Sheet	Random coil	Tm	Kd, app
				°C	m
Wild type	0.25	0.20	0.55	49.7	1.7 ± 1.1 × 10−7
R1433H	0.11	0.39	0.50	NDa	ND
W1408L	0.23	0.24	0.53	57.1	8.1 ± 1.9 × 10−7
C1488R	0.20	0.27	0.53	66.7	6.7 ± 1.4 × 10−7

^a ND means not determined.