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. 2010 Apr 20;285(25):19384–19390. doi: 10.1074/jbc.M110.125377

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — Ole1p-Myc and Ole1p-FLAG association revealed by co-immunoprecipitation analysis. A, yeast extracts from strain YPH499 expressing combinations of Ole1p-Myc and Ole1p-FLAG, as indicated, were subject to immunoprecipitation (IP) with anti-FLAG antibody or lacking the addition of antibody as negative control (NC). Samples were separated by SDS-PAGE, immunoblotted, and visualized with the use of anti-Myc antibodies. The bands corresponding to Myc- and FLAG-tagged Ole1p are indicated. B, same as A except that immunoprecipitation was performed with anti-Myc antibodies and immunoblotting was performed with anti-FLAG antibodies.