FIGURE 3.

Membrane split ubiquitin yeast two-hybrid analysis shows that Ole1p self-associates in vivo. A, schematic representation of the pairs of bait and prey plasmid constructs. The bait Ole1-Cub-artificial transcription factor PLV (protein-LexA-VP16) construct is driven by the met25 promoter, and prey expression is driven by the ADH promoter. PC, positive control; NC1, NC2, and NC3, negative controls; Kan MX, kanamycin resistance module; KAT 1, Arabidopsis K⁺ channel protein; T-H, tail to head; T-T, tail to tail. B, challenge of yeast harboring the constructs illustrated in A on nonselective minimal medium, SD-L-T, (left) and selective minimal SD-T-L-H-A medium (middle) and X-gal assay (right) in which LacZ activity with X-gal as substrate is indicated by dark coloration.