FIGURE 3.

Zn-dependent respiratory defects. In a large-scale screen for mutants showing Zn-dependent respiratory defects, we isolated mzm1Δ from a collection of 684 deletion strains lacking proteins predicted to have mitochondrial localization. Panel A, mzm1Δ cells are unable to grow on respiratory carbon sources under Zn-limited conditions (EDTA) or at elevated temperature relative to both wild type and the complemented strain. Panel B, the presence of m-Adh1 severely restricts mzm1Δ respiratory growth.