FIGURE 6.
pgant3 mutations result in intracellular Tiggrin accumulation. Wild type (A) and pgant3m1/Df(2R)Exel6283 (B) pupal wings at 36 h APF were stained for Tiggrin (Tig) (red) and DE-cadherin (DE-cad) (green). Merged images are shown in yellow. Shown are representative X-Y confocal images (1 μm thick) of pupal wings in the center of the dorsal cell layer (Dorsal) and in the center of the ventral cell layer (Ventral). pgant3 mutants show loss of Tiggrin along cell membranes and accumulation within the cells of the dorsal and ventral cell layers.