FIGURE 2.

MYCN regulates transcription of ABC transporter genes in TET21/N neuroblastoma cells. Modulation of MYCN expression by tetracycline (TET) in TET21/N cells was monitored by Western blot (upper panel). Cluster 3 was used to generate an image map showing expression levels of ABC genes as a function of tetracycline treatment time, relative to expression at time 0 and processed in log space. Hierarchical clustering on each axis used the average-linkage algorithm, and distance measures are based on Pearson correlation. Green indicates genes positively regulated by MYCN, whereas red indicates those negatively regulated (lower panel).