FIGURE 3.

MYCN associates with the promoters of multiple ABC gene family members. A, quantitative ChIP was applied to TET21/N cells expressing MYCN. Fold enrichment is relative to the preimmune serum. The results represent the means ± S.E. of five independent ChIP experiments in which each region was amplified by RQ-PCR in triplicate ± S.E. Bent arrow, transcription start site; gray arrow, canonical E-box; black arrow, noncanonical E-box; open boxes, amplicons indicated with a capital letter. The chromosome and coordinates (bp) are also given (left and middle panel). Dual cross-linking ChIP for the ABCC3 gene promoter using MYCN, MAX, or Sp1 antibodies. The results represent the means ± S.E. of three independent ChIP experiments (right panel). B, quantitative ChIP and quantitative dual cross-linking ChIP was performed for APEX-1 and p21CIP/WAF, respectively.