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. 2010 Apr 13;285(25):19593–19604. doi: 10.1074/jbc.M109.069955

FIGURE 2.

FIGURE 2.

MD-2 gene transfection does not restore LPS-responsiveness to intestinal macrophages. Intestinal macrophages, which are exclusively CD14−, expressed markedly reduced levels of the transcription factors involved in activation of the CD14 promoter (CEBPα, CEBPβ, and Sp1) (A) and low levels of TLR4 protein and no detectable MD-2 protein or MD-2 mRNA (B). MD-2-transfected intestinal macrophages (C, inset) did not release TNF-α, IL-6, or IL-8 after culture for 24 h with rough (R) LPS (1 μg/ml) (C, left panel), whereas a mixture of intestinal macrophages plus 4% blood monocytes released high levels of inducible TNF-α, IL-6, and IL-8 (C, right panel) (values represent mean ± S.E. of triplicate wells). Data are from representative experiments (n = 3) (*, p < 0.005).