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. 2010 Apr 13;285(25):19605–19614. doi: 10.1074/jbc.M109.093864

FIGURE 1.

FIGURE 1.

M10-FULL and M10-ΔFERM fused with GFP. A and B, schematic images of M10-FULL (A) and M10-ΔFERM (B) fused with GFP. C and D, fluorescence confocal images of GFP-M10-FULL (C) and GFP-M10-ΔFERM (D) overexpressed in COS7 cells. Green indicates GFP fluorescent; red indicates actin labeled with Alexa Fluor phalloidin. Scale bars are 10 μm. E, filopodial lengths of cells expressing GFP-M10-FULL (red) or GFP-M10-ΔFERM (blue). The mean filopodial lengths of GFP-M10-FULL- and GFP-M10-ΔFERM-expressing cells were 3.6 ± 1.7 μm (n = 248 in 10 cells) and 2.3 ± 1.4 μm (n = 232 in 12 cells), respectively (p < 0.001 by paired t test). F, average number of filopodia per boundary length of cells expressing GFP-M10-FULL (red) or GFP-M10-ΔFERM (blue). We measured the average number of filopodia for two types of surface coating on the glass bottom dish (Matrigel (MG) and fibronectin (FIB)). The mean filopodial numbers of GFP-M10-FULL- and GFP-M10-ΔFERM-expressing cells on Matrigel coating were 0.23 ± 0.67 filopodia/μm (16 cells) and 0.19 ± 0.63 filopodia/μm (17 cells), respectively (p = 0.22). Those of fibronectin coating were 0.19 ± 0.64 filopodia/μm (18 cells) and 0.22 ± 0.68 filopodia/μm (13 cells), respectively (p = 0.57).