TABLE 1.
GFP-M10-FULL | GFP-M10-ΔFERM | |
---|---|---|
Length of filopodia in steady state (μm) | 3.6 ± 1.7 | 2.3 ± 1.4 |
Average number of filopodia per boundary (number/μm) | ||
Matrigel | 0.23 ± 0.67 | 0.19 ± 0.63 |
Fibronectin | 0.19 ± 0.64 | 0.22 ± 0.68 |
Assembling time (s) | 9.8 ± 5.8 | 11.3 ± 8.2 |
Extension velocity (nm/s) | 69 ± 50 | 124 ± 59 |
79.3a | 128.8a | |
Gaussian peaks (nm/s) | 50 ± 25, 108 ± 54 | 113 ± 56 |
Retraction velocity (nm/s) | −10 ± 17 | −16 ± 20 |
−7.8a | −14.1a | |
Distances of the rapid retraction (nm) | 241 ± 101 | 385 ± 200 |
243a | 391a | |
Maximum velocity of the rapid retraction (nm/s) | 461 ± 277 | 927 ± 390 |
Length of the filopodial extension during each cycle (μm) | 2.4 ± 1.4 | 2.3 ± 1.4 |
Probabilities of second extensions (%) | 25 ± 14 | 5.0 ± 1.0 |
Probabilities of third extensions (%) | 20 ± 20 | 1.7 ± 1.7 |
Duration time until next extension (s) | 85 ± 64 | 69 ± 45 |
Velocity of Intra filopodial movements (nm/s) | 578 ± 210 | 583 ± 212 |
a Obtained from the average trace in Fig. 4.