Table 3.
Promoter Methylation Concordance Between Paired Tissue and Circulating Samples
| Reference | Sample Size | Genes | Methylation Frequency Tissue/BloodB | Coverage A Tissue/Blood | Concordance |
|---|---|---|---|---|---|
| Dulaimi et al (53) | Cases (n=34) |
APC RASSF1A DAPK |
47%/29% 65%/56% 50%/35% |
94%/76% | 81% |
| Controls (n=20) BBD (n=8) |
All genes | 0 | |||
| Sharma et al (70) | Cases (IDC) (n=36) |
p16 p14 CCND2 Slit2 |
44%/36% 47%/36% 27%/25% 58%/58% |
86%/83% | 89% |
| Controls (n=4) |
All genes | 0 | |||
| Mirza et al (71) | Cases (IDC) (n=50) |
TMS1 BRCA1 ESR1 PRB |
24%/24% 26%/22% 66%/48% 64%/46% |
72%/64% | 88% |
| Controls (n=5) |
All genes | 0 | |||
| Shukla et al (72) | Cases (IDC) (n=20) |
RASSF1A RARβ |
85%/75% 10%/0 |
85%/75% | 79% |
| Hu et al (73) | Cases (IDC) (n=36) |
p16 CDH1 |
11%/8% 25%/20% |
31%/25% | 82% |
A
Coverage, percentage of cases having methylation of at least one gene in the given gene panel (i.e. coverage of 100% means that all samples had methylation of at least one gene in the study’s panel); Concordance, between paired samples.
B
In all cases methylation analysis was conducted using methylation specific PCR (MSP).