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. Author manuscript; available in PMC: 2010 Nov 15.
Published in final edited form as: J Immunol. 2009 Oct 28;183(10):6545–6553. doi: 10.4049/jimmunol.0900867

Figure 2. In vitro transcribed dsRNA of specific lengths derived from cloned fragments of the WNv genome is relatively stable within transfected cells.

Figure 2

A, DsRNA of 200 (lane 1), 500 (lane 2), 1000 (lane 3) and 3000 (lane 4) bp lengths was generated using the WNv genome as a template. Approximately 500 ng of each dsRNA was separated on a 1% agarose gel and visualized by ethidium bromide staining. As dsRNA runs slower than DNA, all dsRNA appear to be of the appropriate length when compared to a 1kb DNA marker (lane M). 4 μg of poly IC was also run on the gel (lane 5), with the average length determined to be approximately 4000 bp. B, WT MEFs were mock treated (M) or treated with 1 μg/mL poly IC (pIC) or in vitro transcribed dsRNA of four lengths (ν200, ν500, ν1000 or ν3000) for 6h, after which RNA was extracted. A dsRNA immunoblot was performed using 20μg RNA and dsRNA was detected using the J2 anti-dsRNA antibody. A ladder of 25 ng in vitro transcribed dsRNA was included to determine size (L).