Table 2.
Comparison of photodynamic effects in BMG-1 cells pre-incubated with different concentrations of AlPcS2.
| Endpointa | Time after Photo- irradiation | 0.0 μM | 1.0 μM | 5.0 μM | 10.0 μM |
|---|---|---|---|---|---|
| Intracellular content of AlPcS2 (pg/cell) (absorbance) | 2 h | 0.0 | 1.9 ± 0.05 | 1.9 ± 0.06 | 2.9 ± 0.05 |
| Relative fluorescence intensity | 2 h | 0.0 | 92 ± 13.0 | 105 ± 10.0 | 165 ± 9.0 |
| Fluorescence Distribution | 2 h | - | Perinuclear and Granular in cytoplasm | Diffuse in cytoplasm | Diffuse in cytoplasm but membrane damage in some cells |
| Clonogenic Survival | 240 h | 1.0 ± 0.0 | 0.53 ± 0.07 | 0.65 ± 0.1 | 0.98 ± 0.22 |
| Proliferation Indexb | 42 h | 5.6 ± 0.4 | 3.5 ± 0.5 | 1.0 ± 0.4 | NDc |
| G2+M (%) | 42 h | 7.0 ± 2.0 | 12.0 ± 2.0 | 23.0 ± 0.8 | ND |
| Detached Cells (%) | 42 h | 19.0 ± 2.8 | 15.0 ± 0.3 | 30.0 ± 8.0 | ND |
| Small cells (%) | 42 h | 4.0 ± 0.1 | 24.0 ± 0.2 | 57.0 ± 0.1 | ND |
| Sub G0/G1 (%) | 42 h | 0.0 | 3.0 ± 0.2 | 23.0 ± 10.0 | ND |
| M. F. (%) | 42 h | 1.6 ± 0.2 | 1.5 ± 0.1 | 1.7 ± 0.5 | ND |
aEndpoints are summarized as mean ± standard deviation by groups.
bNt/N0 Where Nt is the cell number at 42 h and N0 is the cell numbers at the time of irradiation.
cND- not determined.