Table 2.
Primers, genomic positions, size of amplified products and annealing temperatures used in the PCR for the different components of the urokinase plasminogen activating system.
| Gene | Primers | Exon | Size (bp) | Tann. |
|---|---|---|---|---|
| uPA | forward 5'-GCCATCCCGGACTATACAGA-3' reverse 5'-AGGCCATTCTCTTCCTTGGT-3' |
8 10 |
417 | 60°C |
| uPAR | forward 5'-CTGGAGCTGGTGGAGAAAAG-3' reverse 5'-TGTTGCAGCATTTCAGGAAG-3' |
3 5 |
406 | 60°C |
| PAI-1 | forward 5'-ATACTGAGTTCACCACGCCC-3' reverse 5'-GTGGAGAGGCTCTTGGTCTG-3' |
3-4 5-6 |
320 | 62°C |
| PAI-2 | forward 5'-GGCCAAGGTGCTTCAGTTTA-3' reverse 5'-GGGATTTTGCCTTTGGTTTG-3' |
2-3 5-6 |
384 | 62°C |
| β-Actin | forward 5'-CAAGAGATGGCCACGGCTGCT-3' reverse 5'-TCCTTCTGCATCCTGTCGGCA-3' |
3 4 |
275 | 62°C |