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(A) Two monospecific antibody preparations (anti-BSA and anti-digoxin) were mixed in vitro at the presence of 0.5 mM reductive GSH. (B) The mixture of anti-BSA and anti-digoxin was injected in a rabbit. Monospecific and bispecific antibody ELISAs were performed to monitor antibody titers at different time intervals after mixing anti-BSA with anti-digoxin in vitro or injecting the mixtures in vivo.
