Skip to main content
. Author manuscript; available in PMC: 2011 Jun 30.
Published in final edited form as: Respir Physiol Neurobiol. 2010 May 12;172(1-2):63–71. doi: 10.1016/j.resp.2010.05.006

Figure 2.

Figure 2

Top: Steady-state levels of TNF-α mRNA induced by E. coli serotype O55:B5 LPS were reduced by brief hypoxia. Total cellular RNA was loaded and run on agarose gels, blotted, and hybridized with murine 32P-labeled specific cDNA probes at the designated time points shown. +, presence; −, absence. Bottom: Densitometry of TNF-α signals was obtained from triplicate samples and normalized to those for glyceraldehyde-3-phosphate dehydrogenase (GAPDH). * P < 0.05 vs. time-matched normoxic LPS control value