Table 1.
GSH Levels Are Unchanged over 6 h in E. coli LPS-Stimulated Alveolar Macrophages with and without Hypoxia and H/R
| Treatment and Time | GSH (nmol/mg cell protein) |
|---|---|
| Baseline pre-LPS, t = 0 | 3.10 ± 0.8 |
| Normoxic LPS, t = 1.5 h | 6.96 ± 3.9 |
| LPS + Hypoxia, t = 1.5 h | 6.32 ± 4.6 |
| Normoxic LPS, t = 2.5 h | 6.24 ± 2.9 |
| LPS + H/R, t = 2.5 h | 5.64 ± 3.3 |
| Normoxic LPS, t = 6 h | 8.36 ± 4.6 |
| LPS + H/R, t = 6 h | 6.14 ± 3.6 |
Abbreviations: GSH, reduced glutathione; H/R, hypoxia/reoxygenation.
Data are means ± SD of duplicate determinations from at least 3 experiments at each time point.