Figure 1. Na⁺ efflux from Na⁺ loaded yeast ²³Na MR spectroscopy.

Panel A: Stacked ²³Na MR spectra obtained from a suspension of Na⁺ loaded yeast in which the SR_e, [TmDOTP_e⁵⁻] was 12.8 mM. The 3 ²³Na resonances are identified as follows (left to right) (1), the extracellular Na (Na_e⁺); (2), the Na standard (Na_std), which contains TmDOTP⁵⁻; and, (3), the intracellular Na (Na_i⁺).

Panel B: The Na⁺ contents, n_Nai (filled square) and n_Nae (open square), of suspensions of Na⁺ loaded yeast (n=4) measured by MRS with SR_e. Mean ± (SD) values are plotted.