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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Cell Death Differ. 2009 Nov 13;17(7):1134–1140. doi: 10.1038/cdd.2009.163

Figure 1.

Figure 1

(a) Structure of l-Ergothioneine: ET is a betaine of the amino acid histidine with a sulfhydryl group attached to the carbon of the imidazole ring. (b) ETT knockdown in HeLa cells. Total RNA was isolated from control and RNAi-transfected cells and 500 ng of RNA was used for the RT-PCR. The top panel shows the RT-PCR product corresponding to the ETT transcript. The bottom panel shows the levels of the GAPDH transcript used as an endogenous loading control. (c) A quantitative representation of the gel shown in B, which shows approximately 75% knockdown of the ETT transcript. (d) [3H]Ergothioneine uptake assay was performed essentially as described earlier. At 72 h post transfection, cells were incubated with 3 µM ET for an hour at 37°C and uptake monitored. The RNAi-depleted cells show about 75% decrease in ET uptake compared with the control cells. The data shown are the mean ± S.E. of three independent experiments ***P<0.001 using the Student’s t-test