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. Author manuscript; available in PMC: 2011 Jul 28.
Published in final edited form as: Neuroscience. 2009 Sep 15;168(4):971–981. doi: 10.1016/j.neuroscience.2009.09.020

Figure 1.

Figure 1

PKC expression and phosphorylation of AQP4. Cell lysates were separated using 10% SDS-PAGE gels and transferred onto PVDF paper and probed with antibodies Western blot analysis was used to examine PKC expression levels in AQP4-D54 cells as compared to primary astrocyte cultures (A) and localization in migrating tumor cells (B) using PKC antibodies as described in Materials and Methods. (C) Immunoprecipitation showed that pretreatment with either chelerythrine or PMA modulated AQP4 phosphorylation levels but did not alter basal AQP4 expression levels (D).