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. Author manuscript; available in PMC: 2011 Jul 28.
Published in final edited form as: Neuroscience. 2009 Sep 15;168(4):971–981. doi: 10.1016/j.neuroscience.2009.09.020

Figure 3.

Figure 3

Role of PKC in modulating migration and adhesion in AQP4-expressing tumor cells. (A) Transwell migration assay comparing AQP4-D54 cells treated with inhibitors of various signaling molecules. 40,000 cells were allowed to migrate for 5 hrs through 8 μm FluorBlok filters. Cells were treated with PKC modulators, chelerythrine and PMA, U73122, a PLC inhibitor and U0126 as a MAPK inhibitor. To measure adherence, 100,000 cells were plated onto various matrices [1% BSA (Control), 10 μg/ml collagen I (CN), 10 μg/ml fibronectin III (FN), 20 μg/ml laminin (LN), 20 μg/ml vitronectin (VN)] for 1 hr. Cells were gently washed away and fixed. Five random images were taken and counted. There was no difference in cell adhesion between D54 cells lacking AQP4 expression (B) nor AQP4-expressing cells (C) when treated with chelerythrine.