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. Author manuscript; available in PMC: 2011 Jun 25.
Published in final edited form as: Brain Res. 2010 Apr 25;1342C:11–23. doi: 10.1016/j.brainres.2010.04.034

Figure 1. Dose-dependent effects of DFP on seizure-like behaviors and the phosphorylation state of DARPP-32 at T75 in C57BL/6 mice.

Figure 1

Female, adult C57BL/6 mice were injected (i.p.) with vehicle (50% DMSO) or DFP in vehicle solution at specific dose levels (2, 3, or 4 mg/kg). Mouse behavior was characterized using a Modified Racine Seizure Inventory every 3 min for 15 min and recorded. The mice were sacrificed by focused microwave irradiation of the head at 15 min post-injection and brain tissue collected for phosphorylation state analysis. (Left panel) Mean seizure ratings are shown for each treatment group (N=5 mice/treatment condition). (Right panel) Mean levels of phospho-T75 DARPP-32 in mouse striatum are shown as a function of DFP treatment. Phospho-T75 DARPP-32 and total DARPP-32 levels were detected in striatal homogenates from individual mice by immunoblotting and antibody binding revealed using dual-channel fluorescence imaging using Li-Cor Odyssey software then normalized for total levels. Data are expressed as a percent ± SEM of levels in the brains of vehicle-treated control mice. (*p<0.05 compared with vehicle-injected controls, ANOVA with Newman-Keuls post-hoc test, n=5 mice/group).