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. Author manuscript; available in PMC: 2011 Jun 25.
Published in final edited form as: Brain Res. 2010 Apr 25;1342C:11–23. doi: 10.1016/j.brainres.2010.04.034

Figure 4. Effect of the muscarinic antagonist, PCH, on phosphorylation of Synapsin I/II on S549 detected after DFP treatment of FVB mice.

Figure 4

Male, adult FVB mice were injected (i.p.) with vehicle solution (50% DMSO) or a 25 mg/kg dose of PCH. Thirty min later mice received an injection (i.p.) or either a vehicle solution or a 4 mg/kg dose of DFP. The mice were then sacrificed by focused microwave irradiation of the head 120 min later. Levels of S549-phosphorylated synapsin I/II and total synapsin I/II were detected were detected by immunoblotting in striatum (left) and hippocampus (right). Antibody binding was revealed using dual-channel fluorescent detection methods. Levels for each phosphoprotein were quantitated using Li-Cor Odyssey software then normalized for total levels. Data are expressed as a percent ± SEM of levels in the striatum of vehicle-treated control mice. (**p<0.01 compared with vehicle-injected controls; ***p<.001 compared with DFP alone, ANOVA with Newman-Keuls post-hoc test, n=5–6 mice/group).