FIG. 5.

The expression of Gfi1-Cre in other tissues and organs. (a–l) After whole-mount X-Gal staining, Gfi1^Cre/+; R26R-lacZ embryos at E13 (a), E15.5 (b–j, l), and P2 (k) were sectioned sagittally (coronally in k) and imaged. Strong lacZ activities are observed in thymus (white arrows in a and b, d), liver (a, b, f), choroid plexus (c, black arrow), dorsal root ganglia of the spinal cord (b, g), skin (b, black arrows in d and g) and heart (d). LacZ activities are also detectable in the urinary organs (asterisk in b, e), gut (white arrowhead in b; black arrowhead in j), pancreas (j, black asterisk), the dorsal epithelium of tongue (i, black arrows), the olfactory epithelia (k), the developing lung (l, arrows), the upper and lower mouth areas (h, black arrows) and in the developing maxillary (h, black arrowheads) and mandibular (h, white arrowheads) areas. (m–p) Immunostaining of the Gfi1-Cre/+; R26R-lacZ/+ retina shows Gfi1-Cre activities (lacZ, green) is confined to the retina ganglion cells marked by anti-POU4F1 (blue) and anti-POU4F2 (red) at E16.5 (m, n) and P14 (o, p). Abbreviations: DRG, dorsal root ganglia; GCL, ganglion cell layer; ht, heart; INL, inner nuclear layer; lv, liver; NBL, neuroblastic layer; ONL, outer nuclear layer; th, thymus. Scale bar equals 50 μm.