Figure 6. LPS- and CpG-induced p65 but not Rel B activity is reduced by TCDD.
Vehicle- or TCDD-treated BMDCs were stimulated with LPS (1 μg/ml) or CpG (0.5μM) for 0.5 hours. Cells were harvested and nuclear proteins isolated. NF-kB p65 and RelB activity was measured using the TransAM activity ELISA. * indicates significant differences between LPS- or CpG-stimulated groups compared to respective vehicle-treated control groups. # represents significant differences between LPS- or CpG-stimulated groups compared to respective untreated control group. Data represents mean ± SEM of 3 samples per treatment group.