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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Arthritis Rheum. 2010 Jun;62(6):1693–1701. doi: 10.1002/art.27408

Figure 5. IFN-α production by pDC requires MHC class II engagement.

Figure 5

pDC (50,000 cells) sorted by flow cytometry were incubated with EBV (15,000 genomes) or HSV-2 (5×10^6 pfu/ml) in the presence or absence of increasing amounts of (A) an anti-HLA DR, DP, DQ or an anti-HLA A, B, C blocking antibody, or (B) an anti-HLA DRα or HLA DRβ chain antibody for 15 minutes. (C) pDC were stimulated with gp42-deficient EBV (15,000 genomes) . After 18 h IFN-α ELISA measured levels in the supernatant. Error bars represent SD for experiments done in triplicate. ND (not detected) represents IFN-α less than 12.5 pg/ml. In (B), statistical analysis was performed by 1-Way ANOVA followed by Dunnett’s comparison. In (C), statistical analysis was performed by Mann-Whitney testing.