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. Author manuscript; available in PMC: 2011 Jul 28.
Published in final edited form as: Neuroscience. 2010 Jan 28;168(4):1019–1035. doi: 10.1016/j.neuroscience.2010.01.037

Fig. 3.

Fig. 3

A) Motor recovery of SCI (n=9) with different severities of initial contusion injury to T10. Mild injuries did not produce immediate and complete paralysis of their hindlimbs (BBB scores were >0), in contrast to moderate or severe SCI. Motor recovery was assessed using the BBB scoring system up to 21d after SCI, when motor recovery is typically finalized in severe or moderate injury. At 21d, SCI rats were sacrificed and the lesion site used for Western blot analysis. Maintaining severely injured rats for longer than 21d poses a problem, because those SCI rats develop severe pain-like behavior in chronically post-injury phase. B) Time course of AQP4 protein level changes in moderate SCI at the lesion site (T10). A representative AQP4 Western blot shows decreases in AQP4 levels at 1, 3 and 14d after SCI (n=3 per time point), and restoration of AQP4 to basal levels by 21d post-SCI. C) AQP4 Western blot (T10, 21d after SCI) in SCI rats with different levels of injury (A) showed that restoration of AQP4 levels happened in mild and moderate SCI, but not in severe, where AQP4 levels remained reduced.