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. Author manuscript; available in PMC: 2011 Jun 16.
Published in final edited form as: J Proteomics. 2010 Apr 12;73(8):1551–1561. doi: 10.1016/j.jprot.2010.03.016

Figure 3. Western blot and SDS-PAGE analysis of sub-cellular fractions.

Figure 3

Lanes are numbered as fractions taken during the fractionation procedure (refer to Figure 2). Samples were load equalized based on total protein. A. Western blot of the fractions. Antibodies used to determine cellular compartments: TM, tropomyosin (sarcomeric); RNApolII, RNA polymerase II (nuclear); SDH, succinate dehydrogenase (mitochondrial); SERCAIIa, sarcoplasmic or endoplasmic reticulum calcium ATPases muscle specific isoform (microsomal); and GAPDH, glyceraldehydes 3-phosphate dehydrogenase (cytoplasmic). B. SDS-PAGE of the same fractions used in the Western blot. STD, molecular weight marker in kDa.