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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Biochim Biophys Acta. 2010 Mar 29;1803(8):960–967. doi: 10.1016/j.bbamcr.2010.03.019

Figure 1. The rate of protein degradation and ubiquitin expression are increased in 21day STZ-treated rat muscle.

Figure 1

(A) Protein degradation was measured in isolated mixed-fiber epitrochlearis skeletal muscles. Muscles from control and 21d STZ-treated rats were incubated in Krebs-Ringer Bicarbonate Buffer with cycloheximide to inhibit protein synthesis. Free tyrosine released into the media was measured fluorometrically after being converted to a nitrosonapthol derivative. (B) Ubiquitin C (rUbC) mRNA in the gastrocnemius muscles was measured by real-time RT-PCR. Data were normalized to 18S RNA and expressed as mean fold increase over control. Data are expressed as the mean ± SEM; n=7/group, P<0.05