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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Exp Neurol. 2010 Mar 15;224(1):170–177. doi: 10.1016/j.expneurol.2010.03.007

Figure 3.

Figure 3

Representative images of the hippocampal CA1 pyramidal layer two weeks after sham or 6 minute TFI. Intrahippocampal injection of AAV2/1 vector expressing shRNA targeting luciferase (luc), calpain 1 (capn1) or calpain 2 (capn2) and co-expressing ZsGreen was performed 2 weeks prior to injury. Sections were imaged for ZsGreen expression and then stained with hematoxylin and eosin (H&E). ZsGreen expression and rounded, basophilic nuclei were seen throughout the CA1 cell layer in sham animals regardless of the vector used, indicative of excellent transduction with all three vectors. After TFI, sections from rats transduced luc or capn2 shRNA had loss of ZsGreen expressing pyramidal neurons and green debris in the neuropile. H&E staining demonstrated nuclear loss and nuclear condensation combined with increased eosinophilic cytoplasm. In contrast, post-TFI sections from rats transduced with capn1 shRNA appeared similar to those from sham injured rats.