Figure 2.
In situ hybridization evidence for the estrogenic modulation of SP and CGRP mRNA expression in the DRG. Sections of rat DRGs were hybridized with specific 33P-labeled riboprobes and autoradiograms were prepared. Top panels show examples of SP mRNA localization in ovx (A) and estrogen-treated (B) groups in bright field photomicrographs of emulsion coated sections. Quantitative data grain counting analyses are shown in the lower graph. Average grain densities (number of grains/µm2) and SEM for small-sized DRG neurons are plotted for the ovx and +E groups with both riboprobes as indicated. The asterisks* indicate significant differences (p<0.05, n= 8) between the two groups for each mRNA probe.
