Table 1.

Primers for PCR of P. chrysosporium genes

Gene*	Forward primer sequence†		Ratio||
1		35	1.3 : 8.7
2		25	2.2 : 7.8
3		30	1.5 : 8.5
4		37	1 : 9
5		27	2.2 : 7.8
6		30	1.5 : 8.5
7		33	1.5 : 8.5
8		31	1 : 9
9		28	1.7 : 8.3

*P. chrysosporium genes (1–9) encoding endopolygalacturonase, exocellobiohydrolase II, cellobiose dehydrogenase, α-galactosidase, cellobiohydrolase, glucan-1,3-β glucosidase, endo-1,4-β xylanase A, β-endoglucanase and Mn peroxidase, respectively.

†Primer sequences are based on sequences from the annotated P. chrysosporium database.

‡Predicted size of PCR product.

§The amplification cycle number was chosen from the linear PCR range for the gene.

||Ratio of 18S rRNA primers and competimers in a duplex PCR reaction.