Fig. 2.

v-slfn effect on NIH3T3 cell growth. (a) NIH3T3 cell line (B7) expressing v-slfn stably upon tetracycline induction was seeded at a density of 5×10⁵ cells per 100 mm dish in triplicate and, after 24 h, expression of v-slfn was induced or not by addition of tetracycline (0.5 μg ml⁻¹). Cells were cultured over 72 h, harvested by trypsinization and counted in duplicate. (b) Cell lysates from (a) were analysed for expression of v-slfn using an α-FLAG mAb (arrow). Data are representative of three independent experiments. Positions of molecular size markers (kDa) are indicated.