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. 2007 Jun;88(Pt 6):1667–1676. doi: 10.1099/vir.0.82748-0

Fig. 3.

Fig. 3.

Characterization of v-slfn by immunoblotting. (a) HeLa cells were either mock-infected (M) or infected with CMLV strain CMS at 5 p.f.u per cell and harvested when indicated. + indicates that AraC was present throughout infection. Cell extracts were analysed by immunoblotting using either α-v-slfn or α-F13 Abs. (b) Expression of v-slfn protein in OPVs. Monolayers of BS-C-1 cells were mock-infected or infected with 5 p.f.u. of the indicated virus per cell, harvested at 6 h p.i. and cell extracts were analysed by immunoblotting using either α-v-slfn or α-F13 Abs. CMLV, camelpox virus; VACV, vaccinia virus; WR, Western Reserve; Patw, Patwadangar; King's Ins, King's Institute; Tashk, Tashkent; COP, Copenhagen; CPXV, cowpox virus; EP2, elephantpox virus-2; BPXV, buffalopox virus; RPXV, rabbitpox virus. (c) HeLa cells were either mock-infected or infected with 10 p.f.u. of the indicated VACV per cell, harvested 16 h p.i. and cell extracts were analysed by immunoblotting. In all panels, the positions of molecular size markers (kDa) are indicated.