Table 1.

Primers used in this study

Primer	Sequence* (5′→3′)	Base-pair positions† at contig
GPA1-5F	CATCTCGGATCTGCCTCTGATT	1439–1460 at 1.228
GPA1-5R	GCACAGGTACACTTGTTTAGAGAGTGACGGTAGTTTGGCTTTGCT	2420–2399 at 1.228
GPA1-3F	CCTTCAATATCACTTCTGTCGAAATGATTTTTTTCTTG	3691–3706 at 1.228
GPA1-3R	GTCTATCGACAGGTCACCGTGT	4683–4662 at 1.228
GPA1-5N	ATTTCTCACTCCCAGCTC	1462–1479 at 1.228
GPA1-3N	GCATGTCTCATCACCAGA	4622–4605 at 1.228
GPA2-5F	GAACGGTCGAGGTCTCTTTGTA	5674–5653 at 1.398
GPA2-5R	GCACAGGTACACTTGTTTAGAGATTGAGAATTAGAAA AA AGCGGC	4700–4721 at 1.398
GPA2-3F	CCTTCAATATCATCTTCTGTCGATAGTGGAACGCTGCTTTTTTCT	3344–3323 at 1.398
GPA2-3R	GCCAACAAGCCATATACCGATA	2386–2407 at 1.398
GPA2-5N	CGCTCCAATTAGTCCATC	5611–5594 at 1.398
GPA2-3N	CACATCATTCGTTCCCAA	2440–2457 at 1.398
GPA3-5F	CCTAGAGAGGTACGCTCCAAAG	55614–55635 at 1.415
GPA3-5R	GCACAGGTACACTTGTTTAGAGAATTGTGTGTTGTTGAAGCGACT	56564–56543 at 1.415
GPA3-3F	CCTTCAATATCATCTTCTGTCGAGATATACGGCGTTTGGACATTT	57978–57999 at 1.415
GPA3-3R	CTTTTGCAGTTTCGTTGTGTGT	58898–58877 at 1.415
GPA3-5N	CCACTGCCAAAAAAACCT	55644–55661 at 1.415
GPA3-3N	GAGCATCAAGCAGACCAG	58872–58855 at 1.415
GPB1-5F	TTTTTGCAGATGCGAAATAGGT	66401–66422 at 1.179
GPB1-5R	GCACAGGTACACTTGTTTAGAGAGCGACAGGAGAGATTAAACGAG	67328–67307 at 1.179
GPB1-3F	CCTTCAATATCATCTTCTGTCGACGTTCACGACCATGAAACTATT	68794–68815 at 1.179
GPB1-3R	TCATCAATGGGATATGCTAAGC	69722–69701 at 1.179
GPB1-5N	GTACATCTAGGCCAGCCA	66469–66486 at 1.179
GPB1-3N	AAAAGACCGAGCAACAGA	69688–69671 at 1.179
Gen-F	CGACAGAAGATGATATTGAAGG	For amplification of gen cassette from pII99 vector
Gen-R	CTCTAAACAAGTGTACCTGTGC

*The underlined sequences are matched with the primer sequences of either Gen-F or Gen-R.

†Based on the F. graminearum genome database.