Figure 3.

MEI4 interacts with REC114. (A) Coimmunoprecipitation of MEI4 and REC114. HeLa cells were transfected with plasmids expressing mouse GST-MEI4 and/or GFP-REC114. Protein extracts were immunoprecipitated with anti-GST or anti-GFP (Supplemental Fig. S5A) antibodies. Inputs (1% of total) and immunoprecipitated fractions (40% of total) were detected with anti-GST or anti-GFP antibodies. Protein standards were used as molecular weight markers (M). (B) Mapping the MEI4 and REC114 interaction domains by yeast two-hybrid assay. The ability to interact of full-length and N-terminal or C-terminal deletion mutants of MEI4 and REC114 was assessed in a yeast two-hybrid assay. Either strong interaction (+++) or no interaction (−) was observed, based on growth on selective media (see also Supplemental Fig. S6).