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Analysis of FIS binding to PguaB by EMSA. EMSA was employed to compare the relative binding affinity of FIS for a DNA fragment containing PguaB (−253 to +36) with an rrnB P1 promoter derivative containing FIS site I (‘rrnB P1+FIS site’) and rrnB P1 containing no FIS sites (‘rrnB P1–FIS site’). The concentration of FIS in each binding reaction is indicated above the corresponding gel lane.
