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. 2008 Jun;154(Pt 6):1729–1738. doi: 10.1099/mic.0.2008/016774-0

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Copyright © 2008, SGM

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3. — Mapping the location of FIS sites at P_guaB by DNase I footprinting. A DNA fragment containing P_guaB (−253 to +36) radiolabelled at the downstream end (relative to the guaB transcription start site), and a DNA fragment containing P_guaB (−133 to +36) labelled at the upstream end, were employed in DNase I footprinting in the presence of different concentrations of FIS (as shown). Nucleotide positions are shown relative to the guaB transcription start site, and lanes containing the G+A ladder are indicated accordingly.