Table 1.

Bacterial strains and plasmids used in this work

Strain or plasmid	Relevant characteristics	Source or reference
A. vinelandii
ATCC 9046	Wild-type	ATCC
SMU88	ATCC 9046 with an algU : : Km mutation	Moreno et al. (1998)
JRA4	ATCC 9046 with mucA : : Gm mutation	Núñez et al. (2000)
SRA4	ATCC 9046 with a mucA : : Km mutation	This work
AQ 20	ATCC 9046 with a fleQ : : Gm mutation	This work
AC 30	ATCC 9046 with a flhC : : Tc mutation	This work
ATCR	ATCC 9046 with a cydR : : Gm mutation	This work
E. coli
DH5α	supE44lacU169hsdR17recA1endA1gyrA96thi1relA1	Gibco-BRL
Plasmids
pMOSBlue	Plasmid used for cloning PCR products	Amersham
pLRQ	pMOSBlue derivative carrying a 2.1 kb DNA fragment containing A. vinelandii fleQ gene amplified by PCR	This work
pLRDC	pMOSBlue derivative carrying a 1.6 kb DNA fragment containing A. vinelandii flhCD genes amplified by PCR	This work
pLRQ30	pLRQ derivative containing a fleQ : : Gm mutation	This work
pLRDC50	pLRDC derivative containing a flhC : : Tc mutation	This work
pMUC	pMOSBlue derivative carrying a 0.91 kb DNA fragment containing A. vinelandii mucA gene amplified by PCR	This work
pSRA4	pMUC derivative containing a mucA : : Km mutation	This work
pMCYDR	pMOSBlue derivative carrying a 1.17 kb DNA fragment containing A. vinelandii cydR gene amplified by PCR	This work
pMCYDR-Gm	pCYDR derivative containing a cydR : : Gm mutation	This work
pJB3Km1		Blatny et al. (1997)
pRK2013		Hedges & Baumberg (1973)
pHP4 Ω-Tc	Source of the Tcr cassette	Fellay et al. (1987)
pBSL141	Source of the Gmr cassette	Alexeyev et al. (1995)
pBSL99	Source of the Kmr cassette	Alexeyev et al. (1995)
pSM40	Source of the Gmr cassette	Peralta-Gíl et al. (2002)
pLRGm-DC	pJB3Km1 derivative carrying a 1.6 kb DNA fragment containing A. vinelandii flhCD genes and the Gmrh gene from pBSL14	This work